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Authors
Affiliations
1 School of Pharmacy Technology and Management, SVKM’s, NMIMS University, Shirpur Campus, Babulde, Near Bank of Tapi River, Shirpur-425405, Dist Dhule (M.S), IN
2 Research and Development Division, Concept Pharmaceuticals Ltd, Aurangabad, IN
Source
Asian Journal of Research in Chemistry, Vol 2, No 3 (2009), Pagination: 280-284
Abstract
A reverse phase high performance liquid chromatographic method was developed for simultaneous determination of Ceftriaxone Sodium and Sulbactam Sodium in bulk and Pharmaceutical Formulation. The separation was done by a Kromasil C18 column (250 mm×4.6 mm, 5 μm) using Acetonitrile: Buffer PH 7: Distilled Water (400:50:550, v/v/v) as mobile phase. The validation of the method was performed, and specificity, reproducibility, precision and accuracy were confirmed. The limit of detection was approximately 0.39 μg/ml for Ceftriaxone Sodium and 0.08 μg/ml for Sulbactam sodium. Due to simplicity and accuracy the method particularly suitable for routine pharmaceutical quality control.
Keywords
RP-HPLC, Ceftriaxone Sodium, Sulbactam Sodium.
